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Image Search Results
Journal: Digital Health
Article Title: Enhancing diabetic retinopathy classification using deep learning
doi: 10.1177/20552076231203676
Figure Lengend Snippet: Evaluation of the system's efficiency against prior studies using the APTOS dataset.
Article Snippet: In addition, Liu et al. employed several TL models including EfficientNetB4, EfficientNetB5, NASNetLarge,
Techniques: Plasmid Preparation
Journal: Endocrinology
Article Title: miR-26a Plays an Important Role in Cell Cycle Regulation in ACTH-Secreting Pituitary Adenomas by Modulating Protein Kinase Cδ
doi: 10.1210/en.2012-2070
Figure Lengend Snippet: Anti–miR-26a induces an accumulation in G1 cell cycle phase. AtT20/D16v-F2 cells were transfected for 48 hours with pre–miR-26a (P), anti–miR-26a (A), pre-miR precursor negative control (cP), and anti–miR-miRNA inhibitor negative control no. 1 (cA). Panel A, Cell-cycle analysis. The graph shows representative data of 3 independent experiments, which were repeated 3 times. Panel B, Representative Western blot showing the protein levels of cyclin E, cyclin A, total JNK, phospho-JNK, total p27, phospho-p27, and β-actin as loading control. Panel C, Representative Western blot showing the protein levels of cyclin E, cyclin A, total JNK, phospho-JNK, total p27, phospho-p27, and β-actin as loading control in AtT20/D16v-F2 cells treated without or with rottlerin (+). Panel D, Representative Western blot showing the protein levels of cyclin E, cyclin A, total JNK, phospho-JNK, total p27, phospho-p27, and β-actin as loading control, in control mock-transfected (−) and in PRKCD-silenced (+) AtT20/D16v-F2 cells. Abbreviation: C, control cells.
Article Snippet: The following primary antibodies were employed: 1/200 rabbit polyclonal antihuman PRKCD (Santa Cruz Biotechnology, Santa Cruz, California), 1/500 rabbit polyclonal antibody to SENP5 (Histo-Line Laboratories, Milano, Italy), 2 μg/mL mouse monoclonal cyclin A (Abcam, Cambridge, United Kingdom), 1/1000 rabbit polyclonal cyclin E (Abcam), 1/2000 rabbit monoclonal antihuman phospho–c-Jun N-terminal kinase (JNK) (Cell Signaling Technology Inc, Danvers, Massachusetts), 1/1000 rabbit polyclonal antihuman total JNK (Millipore, Billerica, Massachusetts), 1/1000
Techniques: Transfection, Negative Control, Cell Cycle Assay, Western Blot, Control